Figure 10.

The metacaspase Yca1p and the serine protease Nma111p play essential pro-survival roles in POA-induced liponecrosis by promoting a proteolytic degradation of aggregated cellular proteins in yeast committed to this PCD. (A) WT, yca1Δ and nma111Δ cells were recovered at day 4 of culturing in YP medium initially containing 0.2% glucose as carbon source. Recovery of insoluble aggregates of denatured cellular proteins and monitoring the extent of protein aggregation were performed as described in Materials and Methods. (B and C) Outlines of the establishment of a pro-death (B) or pro-survival (C) cellular pattern of Yca1p- and Nma111p-dependent protein degradation in yeast committed to POA-induced liponecrosis. See text for additional details. Activation arrows and inhibition bars denote pro-death processes (displayed in red color) or pro-survival processes (displayed in blue color) for liponecrosis elicited by POA. The thickness of activation arrows and inhibition bars correlates with the rates and/or efficacies of the pro-death or pro-survival processes.