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. 2015 Feb 19;14(8):1197–1206. doi: 10.1080/15384101.2015.1013667

Figure 3.

Figure 3.

LIF or bFGF or combination does not activate BMP signaling. Expression of BMP ligands and receptors (A), and BMP downstream genes, ID1-4 (C), was measured by qRT-PCR (*P < 0.05 when compared to the corresponding SHEM group, n = 3) in HCECs at Day 21 cultured in different media. Immunofluorescence staining of pSMAD1/5/8 and pNF-κB was performed to examine the canonical BMP signaling and non-canonical BMP-NFκB signaling in HCECs (B). Scale bars: 25 μm.