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LIF-JAK1-STAT3 signaling in HCECs. The expression of mRNA of JAKs and STAT3 was measured at Day 21 in different cultures by qRT-PCR (A) *P < 0.05 when the corresponding SHEM group as the control; #P < 0.05 when the corresponding ESCM group as the control, n = 3). The immunostaining of Tyr705-phosphorylated form of STAT3 in HCECs cultured in ESCM+LIF and MESCM for 3 h was obtained from the confocal microscope (B). Scale bars: 25 μm.
