Skip to main content
. 2015 Feb 19;14(8):1197–1206. doi: 10.1080/15384101.2015.1013667

Figure 5.

Figure 5.

LIF delays contact inhibition and overexpression of ESC and NC markers. At Day 21, scRNA, JAK1 or STAT3 siRNA was added to the cultures for 48h and BrdU labeling was measured by immunostaining (A). At Day 35, JAK1 or STAT3 siRNA was added to the cultures for 48 h and BrdU labeling was measured by immunostaining (B) *P < 0.05 when compared to the scRNA group). The expression of ESC and NC markers was measured 48 h after JAK1 and STAT3 siRNA knockdown in HCECs cultured in ESCM+LIF (C) *P < 0.05 using ESCM group as the control, #P < 0.05 using ESCM+LIF group as the control) and ESCM+bFGF (D) *P < 0.05 using ESCM group as the control, #P < 0.05 using ESCM+bFGF group as the control) at Day 21 by qRT-PCR. Scale bars: 25 μm.