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. 2015 Feb 19;14(8):1197–1206. doi: 10.1080/15384101.2015.1013667

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© 2015 Taylor & Francis Group, LLC

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Figure 6. — Association of expression of cell cycle related genes and contact inhibition. The mRNA expression of cell cycle related genes at Day 21 (A) and Day 35 (B) were measured by qRT-PCR (*P < 0.05 when the corresponding SHEM group as the control; ^#P < 0.05 when the corresponding ESCM group as the control, n = 3). The immunostaining of p-Rb (Ser807/811) at Day 21 and Day 35 in HCECs cultured in different media (C). The double immunostaining of p-Rb (Ser807/811) and Cyclin E2 (D), E2F2 (E) and p16^INK4a (F) at Day 21 or Day 35 was obtained from the confocal microscope. Scale bars: 25 μm.