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. 2014 Oct 30;13(17):2723–2732. doi: 10.4161/15384101.2015.945828

Figure 1.

Figure 1.

Expression and localization of TGN38 in mouse oocytes during meiotic maturation. (A) Relative level of TGN38 mRNA in mouse oocytes during meiotic maturation. TGN38 mRNA levels were normalized to the maximum levels at GV stage. Samples were collected for quantitative RT-PCR when the oocytes were cultured for 0 h, 8 h or 12 h, corresponding to GV, MI or MII stages, respectively. Each sample contains 50 oocytes. (B) Protein level of TGN38 in mouse oocytes at GV, MI and MII stages. Each sample contains 200 oocytes. (C) Oocytes at GV, MI or MII stages were co-stained anti-TGN38 (green) and anti-γ-tubulin (red) antibodies. Besides the localization in the cytoplasm, TGN38 co-localized with γ-tubulin during oocyte maturation. Bars, 10 μm.