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. 2014 Oct 30;13(17):2723–2732. doi: 10.4161/15384101.2015.945828

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© 2014 Taylor & Francis Group, LLC

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Figure 3. — Depletion of TGN38 in mouse oocytes caused MI arrest and decreased percentage of first polar body extrusion. (A) Relative level of TGN38 mRNA in mouse oocytes after injection of scrambled siRNAs or TGN38 siRNAs. Following injection, oocytes were cultured in M2 medium containing 2.5 μM milrinone for 24 h before collected for quantitative RT-PCR. Fifty oocytes were collected in each sample. *, P < 0.001. (B) TGN38 protein level in control and TGN38 RNAi groups. Each sample contains 200 oocytes. (C) Percentages of oocytes at different stages when cultured for 12 h after TGN38 RNAi. *, P < 0.05. (D) Representative images of BubR1 localization in TGN38 depleted oocytes. Arrow: PB1. Bar, 10 μm.