Figure 2.

Schematic transmembrane (TM) topology of mouse TAAR1 (adapted from the human TAAR1) and frequency of B6- and D2-like Taar1 alleles in MALDR and MAHDR mice. (a) Amino-acid residues incorporated in the transmembrane domains are shaded in gray. Residues comprising the putative ligand binding vector in locations homologous to human TAAR1 are colored red. N-linked glycosylation at N9, as well as the disulfide bridge linking C95 and C181, are indicated according to the annotation in Uniprot entry Q923Y8_TAAR1_MOUSE. Mouse SNP rs33645709 encodes a non-synonymous proline to threonine mutation at amino-acid position 77 (P77T) in D2, compared with B6 mice. Further details are provided in the text. Figure adapted with permission from Lindemann et al (2005). (b) Frequency of B6 and D2 Taar1 alleles in MALDR and MAHDR mice. Taar1 was sequenced in MALDR and MAHDR mice (n=10/line; replicate 2, selection generation 5). ‘A’ and ‘C’ refer to adenine and cytosine, respectively. MAHDR mice are homozygous for the D2 allele at nucleotide 229. This SNP leads to a threonine at amino-acid position 77. MALDR mice are either homozygous or heterozygous for the B6 allele. B6: C57BL/6J; D2: DBA/2J; MALDR: MA low drinking; MAHDR: MA high drinking.