Figure 7.

Loss of Cdk5rap2 does not affect non-neural mESC differentiation into beating cardiomyocytes. (A) Scheme of assay to evaluate mESC differentiation into beating cardiomyocytes according to the validated embryonic stem cell test.²⁴ (B) Normal cardiac differentiation was obtained for a Cdk5rap2 shRNAi mESC clone with strong Cdk5rap2 downregulation (sh1) as well as for control and scramble (n = 3 experiments, 24 wells each, one-way ANOVA, p = 0.1238, Bonferroni's Multiple Comparison Test, ns, not significant). Abbreviations: Co, control; scr, scramble; sh1–4, shRNAi clones 1–4; ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001.