Figure 4.
TUDCA modulation of NSC differentiation-induced mitochondrial alterations is dependent on mtROS and ATP regulation. NSCs were expanded, induced to differentiate for 1 h and 24 h, in the presence or absence of TUDCA and/or CsA or OligA, and then collected for flow cytometry and luminescence detection, as described in Materials and Methods. (A) Representative quantification data of mtROS levels after 1 h of differentiation, using MitoSOXTM Red reagent. (B) Representative quantification data of ATP levels at 24 h of differentiation, using Mitochondrial ToxGloTM assay. (C) Representative quantification of NSC viability by measuring Annexin-V- and PI-negative cells using flow cytometry, at 24 h of differentiation. Results are expressed as mean ± SEM fold-change for at least 3 different experiments. *P < 0.01 and §P < 0.05 from non treated cells (control); ‡P < 0.01 and †P < 0.05 from cells treated with TUDCA alone.