Figure 2. Co-localization of SNAP23 with airway epithelial lineage markers.

A section of mouse lung was probed with primary antibodies from different species and then labelled with species-specific fluorescent secondary antibodies. The low-magnification image (left) shows the epithelium of a small airway with SNAP23 stained red, CCSP stained blue and AcTub stained green. The boxed area is shown at higher magnification with DAPI-labelled nuclei stained grey in the merged image and with each fluorochrome shown separately in grey scale below. The sub-epithelial basement membrane was identified by auto-fluorescence in the green channel and its position is indicated by the yellow dotted line. The nucleus in the submucosa probably belongs to a smooth muscle cell based on its elongated shape and proximity to the epithelium. Scale bar for low magnification image=20 μm and for high magnification images=10 μm.