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. 2015 Jun 12;35(3):e00205. doi: 10.1042/BSR20150047

Figure 1. Fluorescent and immunoblot images of the HKII truncation mutants.

Figure 1

Stably-transfected U-2OS cells were grown in chamber slides with a plasmid carrying FLHKII, a 10aa deletion or a 20aa deletion in the N-terminal end of HKII. The cells were imaged at 400× magnification using an EVOS FL microscope. (A) U-2OS cells transfected with GFP-tagged wild-type FLHKII; (B) U-2OS cells transfected with GFP-tagged HKII (pI2_F10del); (C) U-2OS cells transfected with GFP-tagged HKII (pI2_Q20del); (D) immunoblot analysis of mitochondrial (Mito) and cytoplasmic (Cyto) fractions of U-2OS cells stably-transfected with wild-type FLHKII-GFP, HKII-GFP (pI2_F10del), or HKII-GFP (pI2_Q20del). Immunoblots were probed with primary antibodies to detect: hexokinase II, β-actin and mtHsp70. Densitometry was used to quantify the expression levels of HKII in the cytoplasmic and mitochondrial cell fractions.