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. 2015 Jul 29;35(4):e00229. doi: 10.1042/BSR20150084

Figure 6. NRP1 was the direct target of miR-148b.

Figure 6

(A) The interaction of miR-148b and the 3′-UTR of NRP1. (B) The mutation of miR-148b-binding site of NRP1 3′-UTR. (C) Luciferase reporter assays with co-transfection of WT or MUT 3′-UTR of NRP1 and miR-148b or miR-NC as indicated. Firefly luciferase activity was normalized by Renilla luciferase activity. (D) Western blot analysis of NRP1 protein level in miR-148b overexpressed or knocked-down cells of PLC/PRF/5 and HuH-7. E. qRT-PCR analysis of NRP1 mRNA expression in miR-148b overexpressed or knocked-down cells of PLC/PRF/5 and HuH-7.