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. 2015 Sep 29;35(5):e00251. doi: 10.1042/BSR20150153

Figure 4. Overexpressing human Cx43 proteins in MCF-7 cells.

Figure 4

(A) The schematic diagram of the human Cx43 gene structure containing the 1149-bp CDS Exons and 1723-bp 3′-UTR. (B) The pCDNA-Cx43, a human Cx43 protein overexpression plasmid, was obtained by subcloning the 1149-bp CDS Exons plus 1723-bp 3′-UTR into the pCDNA3.1 vector. The pCDNA-Cx43Δ construct was obtained by subcloning the 1149-bp CDS Exons only into the pCDNA3.1 vector. (C) Immunoblotting assay of human Cx43 in the MCF-7 cells transfected with different doses of pCDNA-Cx43 (0.2 or 0.4 μg/ml), pCDNA-Cx43Δ (0.2 or 0.4 μg/ml) or pCDNA3.1 vector as control for 24 h. (D) Western blotting assay of human Cx43 in the MCF-7 cells co-transfected with pCDNA-Cx43 (0.4 μg/ml) or pCDNA-Cx43Δ (0.4 μg/ml) plus NC (100 nM) or miR-200a (100 nM) for 24 h.