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. 2015 Sep 29;35(5):e00251. doi: 10.1042/BSR20150153

Figure 5. miR-200a/Cx43 axis regulated migration activity of breast cancer cells.

Figure 5

(A) Migration activity of MCF-7 cells transfected with pCDNA3.1 (0.4 μg/ml), pCDNA-Cx43 (0.4 μg/ml) plus NC (100 nM) or pCDNA-Cx43 (0.4 μg/ml) plus miR-200a (100 nM) was measured by transwell assay. (B) Relative transmitted cell numbers in the transwell assay in (A). (C) Transwell assay of MCF-7 cells transfected with pCDNA3.1 (0.4 μg/ml), pCDNA-Cx43Δ (0.4 μg/ml) plus NC (100 nM) or pCDNA-Cx43Δ (0.4 μg/ml) plus miR-200a (100 nM). (D) Relative transmitted cell numbers in the transwell assay in (C). (E) Migration activity of MDA-MB-231 cells transfected with NC (100 nM), miR-200a (100 nM) or miR-1 (100 nM) was measured by transwell assay. (F) Relative numbers of migrated cells in the transwell assay in (E). (B, D and F) Values not sharing a common superscript letter differ significantly (n=5, P<0.05).