Figure 1. Bcnt/Cfdp1 with largely disordered structure.

(A) Molecular architecture of human BCNT/CFDP1. The protein comprises an acidic N-terminal region, lysine/glutamic acid/proline-rich 40 aa stretch (IR) and a highly conserved C-terminal region (BCNT-C). The aa sequences were analysed using UCL software [25]; confidence scores were converted using Excel (Microsoft, Version 14.5.0) to obtain a specific disordered disposition profile with the horizontal axis indicating aa position. • indicates the position of Ser²⁵⁰. A disorder disposition greater than 0.5 in more than 50% of the protein indicates a disorder propensity. The hatched box indicates the 40-aa fragment subjected to NMR analysis. (B) ¹H-¹⁵N HSQC spectrum. NMR analysis was performed on a ¹⁵N-labelled 40 aa fragment of BCNT-C (hatched box).