Figure 6. Phosphatase treatment of the isolated His–BCNT doublet.

Treatment with phosphatases: Isolated His–BCNT from the G11 clone was incubated with the recombinant phosphatases rPPMIA or rPP2A (left panel) or with rPP2A in the presence or absence of okadaic acid (right panel). After 30 min incubation, the samples were boiled in SDS/PAGE sample buffer and subjected to immunoblot analysis with anti-BCNT-C Ab (antibody).