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. 2015 Sep 18;35(5):e00249. doi: 10.1042/BSR20150168

Figure 3. In vitro NAT activity of MBP–zNaa10 and MBP–hNaa10 towards peptide substrates.

Figure 3

The indicated synthetic oligopeptides were incubated with purified MBP–zNaa10 or MBP–hNaa10 enzyme in acetylation buffer at 37°C for 10 min. Product formation using EEEIA (Naa10 substrate) as substrate peptide was defined as 100% enzyme activity for each enzyme. The product formation using DDDIA (Naa10 substrate), SESSS (NatA substrate) and MLGPE (Naa50/NatE substrate) as substrate peptide was correlated to the product formation using EEEIA as substrate peptide. The assays were performed in triplicate (blanks were in duplicate) with error bars indicating S.D.