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. 2015 Aug 20;35(4):e00242. doi: 10.1042/BSR20150017

Figure 2. Effects of insulin on p-AMPK, p-ACC, MAFbx and MuRF1.

Figure 2

Effects of insulin on p-AMPK, p-ACC (A) and mRNA expression of MAFbx and MuRF1 (B) in L6 myotubes. L6 myotubes were pre-treated with AICAR (1 mM), compound C (CC; 20 μM) or not with AICAR and CC for 1 h, followed by addition of insulin (100 nM) for 30 min (A) or 24 h (B). (A) At 30 min, p-AMPK and p-ACC were measured by western blotting, with GAPDH as the internal standard. Shown are representative blots from three independent experiments. (B) At 24 h, extracted RNA from L6 myotubes were assayed with qRT-PCR. Data were normalized to GAPDH and the values for the control group set at 1.0. Data are expressed as means ± S.D. (n=3). *P<0.05 compared with control. #P<0.05 compared with insulin only treatment group.