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. 2015 Aug 20;35(4):e00242. doi: 10.1042/BSR20150017

Figure 3. Effect of Akt inhibitor on inhibition of p-AMPK and p-ACC by insulin.

Figure 3

The effect of Akt inhibitor (Akt inhibitor IV) on inhibition of p-AMPK and p-ACC by insulin in L6 myotubes. L6 myotubes were pre-treated with Akt inhibitor IV (1 μM) for 1 h, followed by addition of insulin (100 nM) or without insulin for 30 min. p-AMPK, p-ACC and phosphor-Akt (p-Akt) were measured by western blotting, with GAPDH as the internal standard. Shown are representative blots from three independent experiments.