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. 2015 Oct 19;35(5):e00257. doi: 10.1042/BSR20150064

Figure 2. Global histone H3K9me2 in cultured MEFs analysed by immunocytochemistry.

Figure 2

The level of global H3K9me2 was detected by binding of FITC-labelled antibodies specific to H3K9me2. (A) Visualization of H3K9me2-immunostained fibroblasts cultured in DMEM/F12 medium and in medium containing BIX-01294 at various concentrations. Green: FITC-labelled H3K9me2; blue: Hoechst-stained nuclei. Scale bar=10 μm. (B) Dimethylation status was calculated as the intensity of FITC labelling divided by the intensity of nuclear labelling with Hoechst 33342.