Table 2. Calculated Rg values for BAPKO_042220–201.
A summary of Rg values for untagged and His-tagged BAPKO_042220–201 calculated using various methods. The Rg was calculated for both samples manually by Gunier approximation and then by Gnom in reciprocal and real space using the full curve.
| Untagged BAPKO_042220–201 | |||
|---|---|---|---|
| 2.5 mg/ml | 3.5 mg/ml | 4.5 mg/ml | |
| Guinier Rg (Å) | 13.5±0.15 | 14.6±0.11 | 12.5±0.39 |
| Reciprocal space Rg (Å) | 15.7 | 14.3 | 14.4 |
| Real space Rg (Å) | 15.7 | 14.3 | 14.5 |
| His-tagged BAPKO_042220–201 | |||
| 3.0 mg/ml | 4.5 mg/ml | 6.0 mg/ml | |
| Guinier Rg (Å) | 14.2±0.13 | 14.1±0.12 | 14.0±0.10 |
| Reciprocal space Rg (Å) | 16.8 | 16.8 | 16.6 |
| Real space Rg (Å) | 16.8 | 16.8 | 16.7 |