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. 2015 Jun 11;35(3):e00203. doi: 10.1042/BSR20150055

Figure 3. ToLFP assay of embryos from N2 and three DNase II mutants.

Figure 3

(A) ToLFP labelling patterns of N2, nuc-1, crn-6 and crn-7 embryos at the 2-fold stage. Green spots are ToLFP positive signals, which are indicated with white arrows. The anterior of the embryo is shown towards the left. Scale bar: 20 μm. (B) The spot size of ToLFP signals in DNase II mutants. The spot sizes were determined by ImageJ software. More than 10 embryos and over 50 signals were scored for each strain at the comma stage. (C) Quantitative analysis of ToLFP signals in three DNase II mutants at the comma, 1.5-fold and 2-fold stages. At least 16 embryos were scored for each stage and strain. Error bars indicate S.E.M., ***P > 0.001. ns, non-significant.