(A) The ToLFP signals of nuc-1 mutants and transgenic worms in whole embryos at the comma, 1.5-fold and 2-fold stages. (B) The ToLFP signals of nuc-1 mutants and transgenic worms in the head region of embryo at the comma, 1.5-fold and 2-fold stages. (C) The ToLFP signals of nuc-1 mutants and transgenic worms in the tail region of embryo at the comma, 1.5-fold and 2-fold stages. The n number of executed embryos in each strain and stage is indicated above the bar. At least 18 embryos were scored for each stage and strain. Error bars indicate S.E.M., *P > 0.05, **P > 0.01, ***P > 0.001. ns, non-significant.