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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Apr 20;35(3):290–300. doi: 10.1038/onc.2015.94

Figure 1. Overview and characterization of XactMice.

Figure 1

(a) Schematic describing the generation of XactMice from human HSPCs, whose progeny migrate into the xenograft and differentiate into stromal cells. The growth and composition of tumors can be compared in nude, NSG, and XactMice. (b) Flow cytometry measuring the expansion of HSPCs in vitro by the percentage of CD34/CD38+ cells. (c) Flow cytometry detecting human hematopoietic CD3/CD45+ cells in peripheral XactMice - but not NSG - blood, indicating that the HSPCs have successfully engrafted and are generating circulating lymphocytes. (d) The average percentage of human CD3/CD45+ cells in the peripheral blood of XactMice, as determined by flow cytometry, over the course of seven months after engraftment. (e, f) There were no significant differences in either CUHN004 or CUHN013 tumor growth rates between nude, NSG, and XactMice. Tumor measurements (W × W × H)/2 were recorded from all mouse strains in three separate experiments. Although tumors seem to grow faster in the NSG and XactMice, no statistical difference was observed in growth between the three strains in these experiments. Average tumor volumes (mm3) with the standard errors were used to create the recorded growth curves.