Skip to main content
. 2015 Oct 20;10(10):e0140968. doi: 10.1371/journal.pone.0140968

Fig 2. Cep169/NCKAP5L is a centrosomal protein with molecular weight of 169 kDa.

Fig 2

(A) IB of whole-cell lysates from HeLa, U2OS, and 293T cells treated with control Luciferase (Luc) siRNA or Cep169/NCKAP5L siRNA, and detected with anti-Cep169 and anti-β-tubulin antibodies. (B) IB of whole-cell lysates transfected with control (Luc siRNA) or two independent siRNA oligonucleotides (Cep169-1 and Cep169-2 siRNAs), probed with antibodies against Cep169 and β-tubulin. (C) Determination of polypeptide molecular size for human Cep169/NCKAP5L by silver staining. The silver staining bands of FLAG-Cep169 observed in the presence of doxycycline disappeared at right lane in the absence of doxycycline. Graph showing estimation of polypeptide size (molecular weight 169 kDa) for Cep169/NCKAP5L. (D) Immunofluorescence images of U2OS cells probed with anti-Cep169 and γ-tubulin antibodies (left). (Scale bar, 10 μm.) U2OS cells were treated with control (Luc siRNA) or Cep169/NCKAP5L siRNA. The centrosomal intensity of γ-tubulin in the U2OS cells (right). (n = 30 cells for each case; error bars, S.E.). (E) Immunofluorescence images of FLAG-Cep169-expressing mitotic HeLa cells probed with anti-FLAG and γ-tubulin antibody. (Scale bar, 10 μm.).