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CDy9 is a novel and versatile mESC-specific fluorescent probe. (a) Chemical structure of CDy9. (b) CDy9 selectively stains mESC over MEF. Both mESC and MEF were incubated with 1 μM CDy9 and imaged under the fluorescence microscope after 1 h. Scale bar: 25 μm. (c) Flow cytometry analysis of mESC and MEF after incubation with CDy9. The fluorescence intensity of mESC upon treatment with CDy9 is brighter than in MEF (right dot plot) and in unstained mESC (left dot plot).
