Skip to main content
. 2015 Nov 1;25(21):4862–4865. doi: 10.1016/j.bmcl.2015.06.037

Figure 2.

Figure 2

Isolation of CDy9-stained cells and analysis by single cell PCR. (a) CDy9 selectively stains mESC in co-cultures with MEF. Co-cultures were incubated with 1 μM CDy9 and imaged under the fluorescence microscope after 1 h. Scale bar: 100 μm. (b) FACS analysis of co-cultured mESC and MEF after CDy9 staining. X-Axis refers to the CDy9 fluorescence intensity and Y-axis refers to the side scattering (SSC), an indication of the size of the cells. FACS enabled the isolation of CDy9-bright and CDy9-dim populations. (c) Table of primers used to distinguish pluripotent (red) from differentiated (blue) cells. (d) Heatmap obtained from single cell PCR analysis of CDy9-bright and CDy9-dim populations. CDy9-bright cells display an up-regulation of pluripotent genes (mESC-like) and CDy9-dim cells show an up-regulation of differentiated genes (MEF-like).