Figure 3.

Diagram of pluripotent stem cell differentiation and cell panel test. (a) mESC are derived from the inner cell mass of a blastocyst and able to differentiate into all cells types of the three primary germ layers: mesoderm (e.g., muscle, blood), endoderm (e.g., pancreas), ectoderm (e.g., neurons). (b) CDy9 staining in different stem cells: mESC, mouse mesenchymal stem cells (mMSC) and mouse neuronal stem cells (NS5). Scale bar: 200 μm. (c) CDy9 staining in different pancreatic cells (endoderm). (d) CDy9 staining in different muscle and blood cells (mesoderm). (e) CDy9 staining in different neuronal cells (ectoderm). In all cases, cells were incubated with 1 μM CDy9 and Hoechst 33342 (nuclear counterstain) and imaged under the fluorescence microscope after 1 h.