Table 1.
Association of RNA expression of circadian genes with metastasis free survival. RNA expression of CLOCK, PER1, PER2, PER3, CRY2, NPAS2 and RORC is significantly associated with better metastasis free survival (MFS) in patients with node-negative breast cancer (N = 766) in the univariate Cox analysis (HR < 1, P < 0.05). In contrast, TIMELESS is associated with worse MFS (HR > 1, P < 0.05, bold face). HR: hazard ratio, FDR: False discovery rate, 95% CI: (95% confidence interval)
| A) E-box regulators: | ||||||
|---|---|---|---|---|---|---|
| Prognostic factor (RNA expression) | Affy probe set | HR (MFS) | P value | FDR | 95%-CI lower upper | |
|
CLOCK |
217563_at |
0.835 |
0.008 |
0.032 |
0.731 |
0.954 |
|
CLOCK |
204980_at |
1.035 |
0.615 |
0.717 |
0.906 |
1.182 |
|
ARNTL |
210971_s_at |
0.908 |
0.159 |
0.262 |
0.793 |
1.039 |
|
ARNTL |
209824_s_at |
0.951 |
0.452 |
0.603 |
0.835 |
1.084 |
|
PER1 |
36829_at |
0.809 |
0.003 |
0.016 |
0.704 |
0.930 |
|
PER1 |
202861_at |
0.810 |
0.004 |
0.016 |
0.703 |
0.933 |
|
PER2 |
208518_s_at |
0.841 |
0.012 |
0.041 |
0.735 |
0.962 |
|
PER2 |
205251_at |
0.874 |
0.040 |
0.112 |
0.769 |
0.994 |
|
PER3 |
221045_s_at |
0.806 |
0.002 |
0.012 |
0.705 |
0.922 |
|
CRY1 |
209674_at |
1.026 |
0.698 |
0.724 |
0.901 |
1.169 |
|
CRY2 |
212695_at |
0.781 |
<0.001 |
0.003 |
0.685 |
0.889 |
|
NPAS2 |
39549_at |
0.952 |
0.486 |
0.619 |
0.830 |
1.093 |
|
NPAS2 |
205460_at |
0.942 |
0.380 |
0.531 |
0.824 |
1.077 |
|
NPAS2 |
205459_s_at |
0.852 |
0.017 |
0.053 |
0.747 |
0.972 |
|
ARNTL2 |
220658_s_at |
0.893 |
0.126 |
0.235 |
0.773 |
1.032 |
|
BHLHE40 |
201169_s_at |
0.891 |
0.092 |
0.197 |
0.779 |
1.019 |
|
BHLHE40 |
201170_s_at |
0.883 |
0.054 |
0.126 |
0.778 |
1.002 |
|
BHLHE41 |
221530_s_at |
0.918 |
0.190 |
0.296 |
0.807 |
1.044 |
|
B) D-box regulators: | ||||||
|
DBP |
209783_at |
1.102 |
0.140 |
0.244 |
0.969 |
1.254 |
|
DBP |
209782_s_at |
0.898 |
0.119 |
0.235 |
0.785 |
1.028 |
|
NFIL3 |
203574_at |
0.972 |
0.680 |
0.724 |
0.851 |
1.111 |
|
C) RORE-box regulators: | ||||||
|
RORA |
210426_x_at |
1.038 |
0.583 |
0.710 |
0.908 |
1.187 |
|
RORA |
210479_s_at |
1.027 |
0.697 |
0.724 |
0.898 |
1.174 |
|
RORB |
206443_at |
1.082 |
0.225 |
0.332 |
0.953 |
1.228 |
|
RORC |
206419_at |
0.800 |
<0.001 |
0.007 |
0.702 |
0.910 |
|
NR1D2 |
209750_at |
0.988 |
0.855 |
0.855 |
0.865 |
1.128 |
|
D) TIMELESS: | ||||||
|
TIMELESS |
215455_at |
1.137 |
0.046 |
0.117 |
1.002 |
1.290 |
| TIMELESS | 203046_s_at | 1.406 | <0.001 | <0.001 | 1.243 | 1.590 |