Figure 4. Gene expression patterns of adrenergic receptors and Ca²⁺ -handling proteins in WT and Nur77-KO hearts.

RT-PCR analysis of ventricular lysates from WT and Nur77-KO mice (n = 6 each). (a) Expression of α- and β-adrenergic receptors did not differ between WT and Nur77-KO. adra1: α1-adrenergic receptor; adrb1: β1-adrenergic receptor; adrb2: β2-adrenergic receptor (b) serca2a was significantly down-regulated in Nur77-KO mice, while all other assessed Ca²⁺ -handling proteins were not differentially expressed. serca2a: sarco/endoplasmic Ca²⁺ ATPase; plb: phospholamban; ryr2: ryanodine receptor 2; cacnb2: l-type Ca²⁺ channel; ncx: Na⁺/Ca²⁺ exchanger; pmca: plasma membrane Ca²⁺ ATPase. Boxplots represent median, inter-quartile range and minimum/maximum values; **p < 0.01.