Fig. 7.

qPCR analysis of cultured human adrenal chromaffin cells. Transcripts for α3, α7, and β4 were the three most highly abundant mRNA species present in cultured adrenal chromaffin cells, followed by α5 and β2. Transcripts for α2, α6, and α10 were weakly detected, whereas those for α4, α9, and β3 were infrequently detected (data not shown). Transcripts for α3 were on average 3.5 ± 0.5 (n = 3) orders of magnitude more abundant than α6. Data were normalized to the expression levels of reference genes using the log₁₀(2^−ΔCq) method, as described in Materials and Methods. The error bars represent the S.E.M. of three experiments using cell cultures of adrenal glands from three individual donors. Statistical significance was determined using an analysis of variance and Bonferroni test (***P < 0.001).