TABLE 3.

Primers for nAChR subunit mRNA, amplicon size, and annealing temperature used in PCR

Target	Primer Sequence	Size	Temp.	Reference
α2	5′-CCGGTGGCTTCTGATGA-3′ (s)	466 bp	55°C	(West et al., 2003)
	5′-CAGATCATTCCAGCTAGG-3′ (as)
α3	5′-CCATGTCTCAGCTGGTG-3′ (s)	401 bp	55°C	(West et al., 2003)
	5′-GTCCTTGAGGTTCATGGA-3′ (as)
α4	5′-GGATGAGAAGAACCAGATGA-3′ (s)	444 bp	58°C	(Carlisle et al., 2004)
	5′-CTCGTACTTCCTGGTGTTGT-3′ (as)
α5	5′-GGCCTCTGGACAAGACAA-3′ (s)	179 bp	60°C	(Liu et al., 2009a)
	5′-AAGATTTTCCTGTGTTCCC-3′ (as)
α6	5′-TCCATCGTGGTGACTGTGT-3′ (s)	413 bp	55°C	(West et al., 2003)
	5′-AGGCCACCTCATCAGCAG-3′ (as)
α7	5′-CTTCACCATCATCTGCACCATC-3′ (s)	308 bp	58°C	(Kurzen et al., 2004)
	5′-GGTACGGATGTGCCAAGGATAT-3′ (as)
α9	5′-GTCCAGGGTCTTGTTTGT-3′ (s)	403 bp	55°C	(West et al., 2003)
	5′-ATCCGCTCTTGCTATGAT-3′ (as)
α10	5′-CTGTTCCGTGACCTCTTCG-3′ (s)	388 bp	60°C	(West et al., 2003)
	5′-GAAGGCCGCCACGTCCA-3′ (as)
β2	5′-CAGCTCATCAGTGTGCA-3′ (s)	347 bp	55°C	(West et al., 2003)
	5′-GTGCGGTCGTAGGTCCA-3′ (as)
β3	5′-TGGAGAGTACCTGCTGTTCA-3′ (s)	439 bp	58°C	(Carlisle et al., 2004)
	5′-CGAGCCTGTTACTGACACTA-3′ (as)
β4	5′-AGCAAGTCATGCGTGACCAAG-3′ (s)	210 bp	60°C	(Liu et al., 2009a)
	5′-GCTGACACCTTCTAATGCCTCC-3′ (as)