Beige fat expansion promotes improved metabolic profiles in C57BL/6J mice. A: Weight gain of mice after 2 weeks of consuming 60% fat diet and being implanted with BAT-MACT or controls receiving cells with no matrix, cell-free matrix, or WAT-MACTs (n = 6). B: Cumulative kilocalories consumed by the animals over 2 weeks. NS, not significant. C: Body weight of animals injected with matrix-free cells or BAT-MACTs consuming a 60% fat diet over the course of 6 weeks (n = 4). D: Serum nonesterified fatty acid (NEFA) concentration. E: Resting blood glucose concentrations. F: Glucose tolerance test area under curve (AUC) (time points: 0, 20, 30, 60, and 120 min) with an identical dose of glucose given to animals after 2 weeks of consuming 60% fat diet and being implanted with either BAT-MACT or matrix-free control (n = 6). Significance at *P < 0.05, **P < 0.01, and ***P < 0.001.