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. 2015 Feb 19;14(8):1164–1173. doi: 10.1080/15384101.2015.1010866

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Sharon Brookes, Sladjana Gagrica, Elaine Sanij, Janice Rowe, Fiona J Gregory, Eiji Hara, and Gordon Peters

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Effects of CDK4 knockdown on the senescence phenotype. (A) Representative images of SVts8 cells transduced with CDK4 or control shRNA at the permissive (34°C) and non-permissive (39°C) temperatures. Cells were stained with DAPI or for CDK4 as indicated. (B) Cell size measurements of SVts8 cells transduced with CDK4 or control shRNA at 34°C or switched to 39°C for 6 days. (C) Cell cycle profiles of SVts8 cells transduced with CDK4 shRNA or control shRNA at 34°C, or switched to 39°C for 5 days.