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. 2015 Jun 9;14(16):2701–2710. doi: 10.1080/15384101.2015.1058677

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© 2015 Taylor & Francis Group, LLC

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Figure 1. — Expression and subcellular localization of Nuf2 during mouse oocyte meiotic maturation. (A) Expression of Nuf2 mRNA as revealed by real time RT-PCR analysis. Samples of 100 oocytes were collected after culture for 0, 4, 8, 9.5 or 12 h, the time points when most oocytes had reached the GV, GVBD, MI, ATI and MII stages, respectively. (B) Confocal microcopy showing the subcellular localization of myc₆-Nuf2 (green) in mouse oocytes at GV, GVBD, pro-MI, MI, ATI and MII stages. DNA (red) was counterstained with Hoechst 33342. Scale bars: 10 µm.