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Overexpressed Nuf2 inhibits MII-AII transition and sister chromatid segregation. (A) MII oocytes were injected with Myc6-control or Myc6-Nuf2 mRNA and cultured for 2h after parthenogenetic activation. DNA (red); α-tubulin (green). Scale bars: 10 µm. (B) Percentages of oocytes arrested at MII in control and overexpression groups. Data are expressed as mean ± SEM of at least 3 independent experiments. *Significantly different (P < 0.05).
