Figure 6.
Overexpressed Nuf2 causes defects of meiotic spindle and chromosome alignment, and prevents the metaphase-anaphase transition of mouse oocytes as revealed by time-lapse live-cell imaging. (A) Oocytes co-injected with β5-tubulin-GFP mRNA and Myc6-control mRNA. Spindle (fluorescent tubulin) and DNA (red) images in a typical control oocyte during in vitro maturation. Time points indicate the time-lapse from about 3-4 h after GVBD. (B) Similar to (A), oocytes were co-injected with β5-tubulin-GFP mRNA and Myc6-Nuf2 mRNA. Shown are images of oocytes with abnormal spindles, aberrant chromosomes, unsuccessful chromosome segregation and failure of PB1 extrusion. Additionally, chromosomes become detached from microtubules during the impaired separation process. α-tubulin (green); DNA (red). Scale bars: 20 µm.