Figure 1.

(See previous page). Effect of eCBs on mekagaryocyte differentiation. (A) MEG-01 cells were left untreated (ctrl) or treated with increasing concentrations (0.1-10 μM) of 2-AG or AEA, or with 10 nM TPA (+), or with 0.1 μM ACEA (CB₁ agonist) or JWH015 (CB₂ agonist); cells were also incubated with 1 μM 2-AG, after pre-incubation with 0.1 μM JZL184, 0.1 μM AM281 or 0.1 μM SR144528 [the last 2 compounds used alone or in combination (mix)]. After 24 hours, the percentage of differentiated cells was counted by an inverted microscope. Values are reported as percentage of control, set to 100% (absolute value = 10.0 ± 0.1% of differentiated cells). * P < 0.001 vs ctrl; # p < 0.01 and ## P< 0.05 vs 2-AG-treated cells. (B) Differential interference contrast micrographs of cells left untreated (ctrl) or treated with 10 nM TPA or 1μM 2-AG for 24 hours. Scale bar, 10 μm. (C) Cell-cycle analysis performed on cells treated as in (B); percentages of cells in G0/G1, S and G2/M phases are given in each panel.