Table 1.
Immuno-phenotypic characteristics of MEG-01-derived platelets.
| Platelets from control cells | Platelets from TPA-treated cells | Platelets from 2-AG-treated cells | Platelets from TPA-treated cells plus collagen | Platelets from 2-AG-treated cells plus collagen | |
|---|---|---|---|---|---|
| Parameter | |||||
| Number | 1 | 10.0 ± 0.8* | 9.2 ± 0.5* | - | - |
| Side scatter (SSC)a | 1 | 1.8 ± 0.1** | 1.8 ± 0.05** | 2.1 ± 0.1§ | 2.2 ± 0.1§ |
| Forward scatter (FSC)a | 1 | 0.6 ± 0.05 | 0.8 ± 0.02 | 0.7 ± 0.05§ | 1.0 ± 0.2§ |
| CD49a | 1 | 2.2 ± 0.1# | 3.3 ± 0.1# | 3.5 ± 0.1§ | 4.0 ± 0.1§ |
| CD61 a | 1 | 3.13 ± 0.1# | 2.1 ± 0.1# | 4.1 ± 0.1§ | 2.7 ± 0.1§ |
Platelet activation was achieved by treatment with 10 μg/ml collagen and analyzed by FACS. Results are expressed as fold over untreated MEG-01-derived platelets, set to 1. a Mean fluorescent intensity. * P < 0.001 vs control; ** P < 0.05 vs control; # P< 0.01 vs control; § P< 0.05 vs the corresponding control in basal conditions.