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. 2015 Feb 2;14(9):1389–1402. doi: 10.1080/15384101.2015.1005993

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Figure 2. — SMYD1 is upregulated during myogenic differentiation. (A and B) Western blot analysis of the indicated proteins in RD18 NpBI-206 and RH4 NpBI-206 cells, treated or not with doxycycline for the indicated days (miR-206 not induced, NI; miR-206 induced, IND). Cells were always kept in high serum (10%). (C) Western blot analysis of the indicated proteins in C2C12 cells grown in proliferation medium (P) and after 3 or 5 days in differentiation medium (D3, D5). (D) Western blot analysis of the indicated proteins in NIH10T1/2 fibroblasts infected with a conditional MyoD-expressing lentiviral vector (NIH NpBI-MyoD), treated or not with doxycycline for 2 days (MyoD not induced, NI; MyoD induced, IND).