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. 2015 Feb 2;14(9):1389–1402. doi: 10.1080/15384101.2015.1005993

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Figure 4. — SMYD1 is essential to implement the myogenic differentiation program. (A) Western blot analysis of the indicated proteins in RD18 NpBI-206 cells infected with a constitutive control (shctrl) or SMYD1-directed (shSMYD1) shRNA, treated or not with doxycycline for the indicated days (miR-206 not induced, NI; miR-206 induced, IND). (B) Representative MHC immunostaining of the cells indicated in (A), treated with doxycycline for 6 days (miR-206 induced). Images were obtained at 10X magnification. (C) Western blot analysis of the indicated proteins in RH4 NpBI-206 cells infected with a constitutive control (shctrl) or SMYD1-directed (shSMYD1) shRNA, treated or not with doxycycline for the indicated days (miR-206 not induced, NI; miR-206 induced, IND). (D) Representative MHC immunostaining of the cells indicated in (C), treated with doxycycline for 6 days (miR-206 induced). Images were obtained at 10X magnification. (E) Western blot analysis of the indicated proteins in C2C12 cells infected with a constitutive control (shctrl) or SMYD1-directed (shSMYD1) shRNA, grown in proliferation medium (P) and after 3 or 5 days in differentiation medium (D3, D5). (F) Representative MHC immunostaining of C2C12 cells indicated in (E) after 5 days in differentiation medium. Images were obtained at 10X magnification. (G) Western blot analysis of the indicated proteins in NIH NpBI-MyoD cells infected with a constitutive control (shctrl) or SMYD1-directed (shSMYD1) shRNA, treated with doxycycline for 2 days (MyoD induced, IND). (H) Representative MHC immunostaining of the NIH NpBI-MyoD cells indicated in (G). Images were obtained at 10X magnification.