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. 2015 Feb 2;14(9):1389–1402. doi: 10.1080/15384101.2015.1005993

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Figure 5. — SMYD1 is present in both the cytoplasm and the nucleus of differentiating cells and its downmodulation impairs transcription of myogenic genes. (A) Western blot analysis of the indicated proteins in cytoplasmic (C) and nuclear (N) extracts of RD18 NpBI-206 cells treated with doxycycline for 3 days (miR-206 induced, IND). (B) Western blot analysis of the indicated proteins in cytoplasmic (C) and nuclear (N) extracts of C2C12 cells after 5 days in differentiation medium (D). (C) Real-time PCR analysis of the indicated transcripts in total RNA extracted from RD18 NpBI-206 cells infected with a constitutive control (shctrl) or SMYD1-directed (shSMYD1) shRNA, treated or not with doxycycline for the indicated days (miR-206 not induced, NI; miR-206 induced, IND). (D and E) Real-time PCR analysis of the indicated transcripts in total RNA extracted from C2C12 cells infected with a constitutive control (shctrl) or SMYD1-directed (shSMYD1) shRNA, grown in proliferation medium (P) and after 3 or 6 days in differentiation medium (D3, D6).