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. 2015 Feb 2;14(9):1389–1402. doi: 10.1080/15384101.2015.1005993

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Figure 8. — DCA inhibits ERMS and ARMS cell proliferation and sustains myogenic differentiation upon miR-206 withdrawal in ERMS cells. (A) MTT analysis of RD18 cells treated or not with 10 mM DCA for the indicated days. The number of cells at day 0 was set at 100%. (B) MTT analysis of RH4 cells treated or not with 10 mM DCA for the indicated days. The number of cells at day 0 was set at 100%. (C) MTT analysis of RD18 NpBI-206 cells treated or not with 10 mM DCA, in presence of doxycycline (miR-206 induced, IND) or after doxycycline withdrawal (miR-206 de-induced, DEIND) for the indicated days. The number of cells at day 0 was set at 100%. (D) MTT analysis of RH4 NpBI-206 cells treated or not with 10 mM DCA, in presence of doxycycline (miR-206 induced, IND) or after doxycycline withdrawal (miR-206 de-induced, DEIND) for the indicated days. The number of cells at day 0 was set at 100%. (E) Flow cytometry quantification of MHC expression in RD18 NpBI-206 cells treated or not with 10 mM DCA, in presence of doxycycline (miR-206 induced, IND) or after doxycycline withdrawal (miR-206 de-induced, DEIND) for the indicated days.