Figure 2.

The SUMOylation of TopoIIα-CTD is not required for the SUMOylation-dependent inhibition of decatenation activity. (A) Both unSUMOylated (−) and SUMOylated TopoIIα (+) WT, K660R, 3KR, and 4KR were incubated with kinetoplast DNA (kDNA) for the indicated time. The reactions were resolved in an agarose gel to separate decatenated (bracket) from catenated DNA (arrow). (B, C) The band intensity of catenated kDNA from 3 independent experiments was measured as described in the Materials and methods. The relative amount of remaining catenated kDNA is presented as % catenated kDNA (F), and the inhibition rate by SUMOylation of each reaction was calculated by (% catenated DNA in +SUMO reaction)/(% catenated DNA in -SUMO reaction) in each assay with standard error.