Table 1. List of antibodies for immunohistochemistry.

Brain region	Primary antibody	AP coordinates of the sections	Source of antibody	Antibody dilution	Secondary antibody (biotinylated)	Source of antibody	Antibody dilution
To quantify extent of lesion:
Medial septum	Mouse monoclonal anti-parvalbumin (PV) Detects ~12 kDa parvalbumin protein	~1.20–0.00	Sigma, USA P3088 Lot #035M4879V	1:2000	Goat anti-mouse IgG	Calbiochem, USA	1:400
	Rabbit polyclonal anti-choline acetyltransferase (ChAT) Detects ~70 kDa choline acetyltranferase protein in cholinergic neurons	~1.20–0.00	Cedarlane, Canada CLN118 Lot # LH812Y	1:1600	Goat anti-rabbit IgG	Calbiochem, USA or Sigma, USA	1:1000
Amygdala	Mouse polyclonal anti-rat CD11b Detects the receptor for the iC3b component of complement in macrophages, dendritic cells, granulocytes and microglial cells in brain	~−2.20–−3.60	AbD Serotec, UK MCA275G	1:1000	Horse anti-mouse IgG	Vector Laboratories, USA	1:1000
To investigate effects of MS lesion on cellular responses:
Rostral anterior cingulate cortex	Rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) Detects dually phosphorylated Erk1/2 at Thr 202 & Tyr204/Thr185 & Tyr187	~5.20–2.20	Cell Signaling, USA #4370 Ref # 05/2014	1:1500	Goat anti-rabbit IgG	Calbiochem, USA or Sigma, USA	1:1000
Amygdala Hippocampus	Rabbit polyclonal anti-early growth response 1 (Egr1/Zif268) Detects ~82 kDa Erg-1 epitopes on C-terminus	~−2.20–−3.60 ~−2.00–−4.50	Santa Cruz, USA sc-110 Lot # K1714	1:1000