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. 2015 Apr 22;138(6):1598–1612. doi: 10.1093/brain/awv092

Figure 2.

Figure 2

Netrin 1 enhances endothelial barrier properties in vitro and in vivo. Permeability coefficient of bovine serum albumin (BSA) (A) and dextran (D10) (B) across primary cultures of human brain-derived endothelial cells, either untreated/resting (R), or treated with astrocyte conditioned media, or recombinant netrin 1 (N1). In situ immunostainings (C) for blood-derived plasma proteins apolipoprotein B (ApoB), Immunoglobulin G (IgG), and fibrinogen, in netrin 1 knockout mice (ko) compared to wild-type mice (wt). Inserts represent magnifications of fibrinogen (green) extravasation in microvessels from wild-type and knockout animals, respectively. Immunostainings for PECAM, tomato lectin and laminin serve to identify vessels. Data shown are the mean ± SEM from n ≥ 5 independent experiments and of n = 12 sections from four animals per group. Plasma protein extravasation was quantified (D–F) by multiplying the mean pixel fluorescent intensity of serum protein and the area of leakage. Scale bars = 20 μm. (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001).