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Multilineage differentiation capacity of the EGFP-HAC ES cells within teratomas, exemplified by the formation of ciliated epithelium (ectoderm germ layer), skeletal muscles (mesoderm germ layer), and gut epithelium (endoderm germ layer), all showing EGFP expression following immunofluorescent staining (lower row). H&E – hematoxylin-eosin staining (upper row).
