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. 2014 Oct 29;13(20):3249–3259. doi: 10.4161/15384101.2014.953879

Figure 1.

Figure 1.

HPV-16 E7 abrogates DNA damage-induced G1 checkpoint in immortalized human epithelial cells. (A) Expression of E7 and pRb. The steady-state levels of E7 and pRb proteins in NIKS and RPE1 cells were determined by immunoblotting. Actin-β was used as a loading control. (B) E7 abrogates DNA damage-induced G1 checkpoint in NIKS cells. HPV-16 E7-expressing and vector-containing NIKS cells were treated with bleomycin, labeled with BrdU, stained with anti-BrdU antibody, counterstained with PI, and analyzed by flow cytometry. Representative flow cytometry of BrdU staining profiles and histograms are shown (Left panel). The percent of BrdU-positive cells is indicated. Mean percent of BrdU-positive NIKS cells from 3 experiments are summarized (Right panel). Error bars reflect the standard deviations of the mean. (C) HPV 16 E7-expressing and vector control RPE1 cells were treated with bleomycin and analyzed as in (B). Representative flow cytometry of BrdU staining profiles and histograms are shown (Left panel). The percent of BrdU-positive cells is indicated. Mean percent of BrdU positive RPE1 cells from 2 experiments are summarized (Right panel). Error bars reflect the standard deviations of the mean.