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. 2014 Oct 29;13(20):3249–3259. doi: 10.4161/15384101.2014.953879

Figure 3.

Figure 3.

B-Myb is important for E7 to up-regulate Cdk1 and abrogate G1 checkpoint. (A) Lysates from NIKS (Left panel) and RPE1 (Right panel) cells expressing E7 or containing vector treated with bleomycin or PBS were assessed for steady-state level of B-Myb by immunoblot. Actin-β was used as a loading control. (B) NIKS (Left panel) and RPE1 (Right panel) cells expressing E7 were transfected with siRNAs targeting B-Myb. The steady-state levels of B-Myb and Cdk1 were determined by Western blot. Actin-β was used as a loading control. A representative of 3 independent experiments is shown. (C) RPE1-E7 and the vector control cells were transfected with B-Myb siRNA, treated with bleomycin and analyzed by flow cytometry after PtdIns staining. A representative experiment of 4 is shown in the left panel. Data of 4 experiments are summarized (Right panel).